Anti -Cyclin D1 (G1/S-specific Cyclin-D1, B-cell Lymphoma 1 Protein, BCL-1, BCL-1
Oncogene, PRAD1 Oncogene, CCND1, BCL1, PRAD1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
B IP IH IC IF |
|
| Cyclins are proteins that regulate cell cycle progression by interacting with cdc2 kinase and/or related kinases. Human cyclins have been grouped into 5 types, A through E, based largely upon sequence similarity. Besides serving as cell cycle regulators, D-type cyclins have been reported to be able to interact with several transcription factors and modulate their transcriptional activations. Cyclin D1, a member of the D-type cyclins, is an important regulator of G1 to S-phase transition in numerous cell types from diverse tissues. Binding of cyclin D1 to its kinase partners, the cyclin dependent kinases 4 and 6 (CDK46) results in the formation of active complexes that phosphorylate the Retinoblastoma tumor suppressor protein (RB). It is an oncogene amplified or over-expressed in more than 50% of all breast cancers. Recent work reveals the role of Cyclin D1 in regulation of cellular metabolism, fat cell differentiation and cellular migration. It maps to 11q13 region of human chromosome. | | | Catalog # | C8454-02J | | Applications | Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:50-1:100 | | Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50 | | Immunoprecipitation: 1:50 | | Optimal dilutions to be determined by the researcher. | | Positive Control | Breast carcinoma | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 11E133 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid, 0.05% sodium azide. | | Purity | Purified | | Immunogen | Recombinant corresponding to human cyclin D1 protein. Cellular Localization: Nuclear. | | Specificity | Recognizes human Cyclin D1. Species Crossreactivity: monkey, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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