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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH IF

Cyclin D1or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. It is a putative protooncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL).
Catalog #	C8454-03A
Epitope	C-terminus.
Applications	Immunofluorescence
	Immunoprecipitation: (Use Protein A) (Ab 10ug/mg protein lysate)
	Western Blot: (Ab 5ug/ml for 2hrs at RT)
	Immunohistology (Formalin/paraffin): (Ab 10-20ug/ml for 30 min at RT) Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Positive Control	MAD109 cells. Breast carcinoma or mantle cell lymphoma.
Cellular Localization	Nuclear.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Isotype	IgG
Concentration	~1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	A synthetic peptide derived from the C-terminal of human cyclin D1.
Specificity	Recognizes Cyclin D1. Species Crossreactivity: Human, Mouse and Rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.