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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin D1 (bcl 1)

Anti -Cyclin D1 (bcl 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Cyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL).
Catalog #C8454-03B
ApplicationsSuitable for ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml for 2 hours at RT.
Immunohistology (Formalin/paraffin): 1:100 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control Breast carcinoma or mantle cell lymphoma.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide from C-terminus of human cyclin D1. Cellular Localization: Nuclear.
SpecificityRecognizes human Cyclin D1 at 36kD. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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