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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH

Cyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL).
Catalog #	C8454-03B
Applications	Suitable for ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 1ug/ml for 2 hours at RT.
	Immunohistology (Formalin/paraffin): 1:100 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
	Optimal dilutions to be determined by the researcher.
Positive Control	Breast carcinoma or mantle cell lymphoma.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%.
Purity	Purified by immunoaffinity chromatography.
Immunogen	A synthetic peptide from C-terminus of human cyclin D1. Cellular Localization: Nuclear.
Specificity	Recognizes human Cyclin D1 at 36kD. Species Crossreactivity: mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.