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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin D1 (bcl 1) (BSA & Azide Free)

Anti -Cyclin D1 (bcl 1) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IF
Cyclin D1 gene is identical with the BCL-1 and the PRAD-1 oncogene and is part of the chromosome 11q13 amplicon which is amplified in several common tumor types like breast, lung, esophagus, urinary bladder and head and neck carcinoma. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. About 50-70% of mantle cell lymphomas (MCL) contain a t (11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. Cyclins were originally identified as proteins whose levels fluctuated during the cell cycle. Subsequent studies have shown the cyclins control entry into different phases of the cycle cell by regulating the activity of cyclin-dependent kinases (CDKs). Mitotic or G2 cyclins regulate entry into M phase, whereas G1 cyclins regulate entry into S phase. Cyclin D1, a G1 cyclin, is essential for control at the G1/S transition (START in yeast), as it regulates the activity of CDK4 and CDK6 protein kinases. Translocation of the gene encoding cyclin D1, the bcl-1 oncogene, is associated with human B-lymphocytic malignancy, suggesting that alteration of cell cycle progression may be oncogenic.
Catalog #C8454-03X
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Kinase Assay. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (Native and denatured): 10ug/mg protein lysate. Co-precipitates Cdk4.
Western Blot: 5-10ug/ml for 2hrs at RT
Optimal dilutions to be determined by the researcher.
Positive ControlMAD109 cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Without BSA and sodium azide. Also available with BSA and Sodium Azide. See C8454-03.
PurityPurified by Protein A affinity chromatography.
ImmunogenSythetic peptide corresponding to aa 285-295 (Cys-TPTDVRDVDI) from the C-terminal of human cyclin D1 (KLH). Cellular Localization
SpecificityRecognizes human cyclin D1 (Mr 36kD) and shows no crossreaction with cyclin D2 or D3. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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