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You are here:Home » Antibodies » Antibodies-Cyclin » Anti -Cyclin D

Anti -Cyclin D


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IC
Cyclins were originally identified as proteins whose levels fluctuated during the cell cycle. Subsequent studies have shown that the cyclins control entry into different phases of the cell cycle by regulating the activity of cyclin-dependent kinases (CDKs). Mitotic or G2 cyclins regulate entry into M phase, whereas G1 cyclins regulate entry into S phase. Cyclin D1, a G1 cyclin, is essential for control at the G1/S transition (START in yeast), as it regulates the activity of CDK4 and CDK6 protein kinases. Translocation of the gene encoding Cyclin D1, the bcl-1 oncogene, is associated with human B-lymphocytic malignancy, suggesting that alteration of cell cycle progression may be oncogenic.
Catalog #C8454-05
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunoblot Analysis 2: At 0.52ug/ml, this antibody detected Cyclin D in a RIPA lysates of human A431 cells, mouse 3T3 fibroblasts.
Immunoprecipitation: 5ug of antibody immunoprecipitated Cyclin D from a RIPA lysate of human A431 cells. An H1 kinase assay can be performed with the immunoprecipitate, as reported previously.
Immunocytochemistry: At 10ug/ml, a previous lot of antibody detected Cyclin D in cells fixed with 4% paraformaldehyde/0.1% Triton X-100. Immunoblot Analysis EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Cyclin D (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Positive Antigen Control EGF-stimulated A431 cell lysate. Use 20ug per lane.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a solution in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, with 0.05% sodium azide.
PurityProtein A purified
ImmunogenSynthetic peptide corresponding to the 11 C-terminal amino acids (residues 285295; sequence: CTPTDVRDVDI) of human Cyclin D1.
SpecificityRecognizes p36 Cyclin D1 and crossreacts with p34 Cyclin D2, which has a highly homologous epitope. No crossreactivity with Cyclin A or Cyclin B. Shows reactivity with a 28kDa band that can competed away by pre-incubating antibody with the peptide immunogen. Also detect a nonspecific band at 21kDa. Species crossreactivity: mouse and rat.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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