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You are here:Home » Antibodies » Antibodies-Cyclin » Anti -Cyclin D

Anti -Cyclin D


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Clone Host Grade Applications
Monoclonal Mouse Ascites B IP
Activity of the cyclin-dependent kinases, cdk4 and cdk6, is regulated by T-loop phosphorylation, by the abundance of their cyclin partners, the D-type cyclins, and by association with cdk inhibitors of the Cip/Kip or INK family of proteins (1). In growing cells, the inactive ternary complex of cyclin D/cdk4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to effect progression through the restriction point in a pRb-dependent entry into S-phase (2). The active complex of cyclin D/cdk4 targets the retinoblastoma protein for phosphorylation allowing the release of E2F transcription factors that activate G1Sphase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of its protein expression and through phosphorylation-dependent degradation (4). Of the three D cyclins, aberrant expression of cyclin D1 has been associated with many forms of cancer including B cell lymphomas and can directly contribute to oncogenesis by various mechanisms including gene translocation or amplification (2). Cyclin D1 also plays a critical role in mammary tissue maturation (5).
Catalog #C8454-05N
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:2000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No3i11
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
ImmunogenRecombinant protein corresponding to aa151-170 of human cyclin D1.
SpecificityRecognizes endogenous levels of total human cyclin D1. Does not crossreact with cyclin D2 or D3. Species crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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