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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP IH

Cyclin D1 is a 36kD nuclear protein. The cell cycle in eukaryotic cells is mediated by the formation, activation, and deactivation of complexes containing Cyclin-Dependent Kinases (CDK) and cyclins. D-type cyclins usually associate with CDK4 (p34 PSK-J3). Complexes with CDK2 and CDK5 have also been reported (1). D-type cyclins are necessary for cells to achieve the G1 to S transition in the cell cycle (2). The D-type cyclins have been shown to associate with PCNA, the retinoblastoma gene product, p21, and other proteins.
Catalog #	C8454-06A
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 0.1-1.0ug/ml
	Western Blot: 0.3-1.0ug/ml
	Immunoprecipitation: 5ug
	Immunohistochemistry: 2-10ug/ml. Formalin-fixed paraffin embedded sections require a Heat Induced epitope Retrieval (HIER) step prior to staining.
	Optimal dilutions to be determined by the researcher.
Source	Ascites
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	3G112
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Full length human cyclin D1 protein.
Specificity	Recognizes cyclin D1. Does not crossreact with other cyclin proteins including other D type cyclins. Does not co-immunoprecipitate CDK4 kinase activity associated with cyclin D1. Species Crossreactivity: Human and mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.