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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IH

Cyclin D1 is a 36kD nuclear protein. The cell cycle in eukaryotic cells is mediated by the formation, activation and deactivation of complexes containing Cyclin-Dependent Kinases (CDK) and cyclins. D-type cyclins usually associate with CDK4 (p34PSK-J3); however, complexes with CDK2 and CDK5 have also been reported (1). D-type cyclins are necessary for cells to achieve the G1 to S transition in the cell cycle (2). The D-type cyclins have been shown to associate with PCNA, retinoblastoma gene product, p21 and other proteins (3,4,5).
Catalog #	C8454-07
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 1:100; Epitope Retrieval with EDTA, pH 8.0 is required. Enzyme digestion is not required.
	Optimal dilutions to be determined by the researcher.
Positive Control Tissue	Mantle cell lymphoma
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	3G113
Host	Mouse
Source	Human
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Purity	Purified
Immunogen	Recombinant full-length human Cyclin D1 protein.
Specificity	Recognizes human Cyclin D1. Expected Staining Pattern: Nuclear.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.