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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin D1

Anti -Cyclin D1

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IP IH IF
Cyclin D1 is a 36kD nuclear protein. The cell cycle in eukaryotic cells is mediated by the formation, activation, and deactivation of complexes containing Cyclin-Dependent Kinases (CDK) and cyclins. D-type cyclins usually associate with CDK4 (p34 PSK-J3 ); however, complexes with CDK2 and CDK5 have also been reported. (2) D-type cyclins are necessary for cells to achieve the G1 to S transition in the cell cycle. (3) The D-type cyclins have been shown to associate with PCNA, retinoblastoma gene product, p21, and other proteins.
Catalog #C8454-09
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.251.0ug/ml
Immunoprecipitation (Active kinase): 25ug
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3G115
HostMouse
SourceMouse
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified
ImmunogenRec-GST fusion protein
SpecificityReacts with mouse cyclin D1 and supports kinase acitivty. Good reactivity with rat cyclin D1 is also observed. USAGE Optimal concentrations of this antibody should be determined by the researcher for each speci c application. Immunoblotting (1)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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