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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin D2

Anti -Cyclin D2

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified B IP IC FC IF
Catalog #C8454-12
ApplicationsSuuitable for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western Blot. Also suitable for neutralization of cyclin D2 activity in vivo (use C8454-12X (azide-free version)).
Recommended DilutionImmunoprecipitation: Use Protein A; 2ug antibody/mg protein lysate
Optimal dilutions to be determined by the researcher.
Positive ControlU-2-OS, C82, RD, Bristol-8 or LoVo36 cells.
Note that T47D, ZR75, SKBR3, MCF-7, BT-20, MDA468, HBL100, BT5549, HeLa, Saos2 and HT29 cells are negative for cyclin D2.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No0.N.240
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.
Also available without BSA and azide.
PurityPurified by Protein A affinity chromatography
ImmunogenPurified human recombinant full-length cyclin D2 protein
SpecificityRecognizes human Cyclin D2 at 34kDa. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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