| Catalog # | C8454-12X |
| Applications | Suuitable for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western Blot. Also suitable for neutralization of cyclin D2 activity in vivo. |
| Recommended Dilution | Immunoprecipitation: Use Protein A; 2ug antibody/mg protein lysate |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | U-2-OS, C82, RD, Bristol-8 or LoVo36 cells. |
| Note that T47D, ZR75, SKBR3, MCF-7, BT-20, MDA468, HBL100, BT5549, HeLa, Saos2 and HT29 cells are negative for cyclin D2. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a,k |
| Clone No | 0.N.240 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 10mM PBS, pH 7.4. |
| Also available with BSA and azide. See C8454-12X. |
| Purity | Purified by Protein A affinity chromatography |
| Immunogen | Purified human recombinant full-length cyclin D2 protein |
| Specificity | Recognizes human Cyclin D2 at 34kDa. Species Crossreactivity |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
