Anti -Cyclin D3 (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S specific cyclin E,
CCND3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IC FC |
|
| Cyclin D3 is a member of Cyclin D family which mediates cell cycle progression through the G1 phase. Cyclin D family binds and activates cyclin-dependent kinases 4 and 6 CDK4, CDK6), which regulates the phosphorylation of retinoblastoma (Rb) protein, p107, and p130 (1). Cyclin D3, similar to cyclin D1 and D2, regulates the initial G1 to S transition by inhibiting Rb and activating E2F proteins (2). Cyclin D3 expression has been linked to inhibition of granulocyte differentiation and activation of various transcription factors, such as transcription factor 5 (3, 4). Overexpression of cyclin D3 is associated with poor clinical outcome in diffuse large B-cell lymphoma cases (5). | | | Catalog # | C8454-17A | | Applications | Suitable for use inFlow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:5000 | | Immunocytochemistry: 1:100-250 | | Flow Cytometry: 1:30 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9E241 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.18M sodium chloride, 40% glycerol, 0.25% sodium azide, 0.25mM EDTA, 25mM Citric Acid, 0.5% BSA. | | Purity | Supernatant | | Immunogen | A synthetic peptide corresponding to a residue near the C-term of human Cyclin D3. | | Specificity | Recognizes human Cyclin D3. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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