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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin D3 (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S specific cyclin E, CCND3)

Anti -Cyclin D3 (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S specific cyclin E,
CCND3)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC IF N
Cyclin D3 is a G1 cyclin closely related to cyclin D1 and D2. Cyclins D1, D2, and D3 form active kinase complexes in vivo with a distinct subset of cdks, particularly with cdk4 and cdk6.
Catalog #C8454-20
ApplicationsSuitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (Native only; Use Protein G): 2ug/mg protein lysate
Western Blot: 1-2ug/ml for 2 hrs at RT.
Immunohistochemistry (formalin/paraffin): 1:25 for 60 min at RT.
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by colling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlLS174T cells, breast carcinoma or tonsil.
Cellular LocalizationNuclear
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.242
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Also available without BSA and azide.
PurityPurified by Protein G chromatography.
ImmunogenPurified human recombinant full length cyclin D3 protein
Cellular LocalizationNuclear
SpecificityRecognizes Cyclin D3 at 34kD. Species Crossreactivity: Human, monkey, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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