Anti -Cyclin D3 (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S specific cyclin E,
CCND3)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH FC IF N |
|
| Cyclin D3 is a G1 cyclin closely related to cyclin D1 and D2. Cyclins D1, D2, and D3 form active kinase complexes in vivo with a distinct subset of cdks, particularly with cdk4 and cdk6. | | | Catalog # | C8454-20 | | Applications | Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. | | Recommended Dilution | Immunoprecipitation (Native only; Use Protein G): 2ug/mg protein lysate | | Western Blot: 1-2ug/ml for 2 hrs at RT. | | Immunohistochemistry (formalin/paraffin): 1:25 for 60 min at RT. | | Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by colling at RT for 20 min. | | Optimal dilutions to be determined by the researcher. | | Positive Control | LS174T cells, breast carcinoma or tonsil. | | Cellular Localization | Nuclear | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 0.N.242 | | Host | Mouse | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. | | Also available without BSA and azide. | | Purity | Purified by Protein G chromatography. | | Immunogen | Purified human recombinant full length cyclin D3 protein | | Cellular Localization | Nuclear | | Specificity | Recognizes Cyclin D3 at 34kD. Species Crossreactivity: Human, monkey, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
