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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin D3 (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S specific cyclin E, CCND3)

Anti -Cyclin D3 (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S specific cyclin E,
CCND3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse B IH
Activity of the cyclin-dependent kinases cdk4 and cdk6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with cdk inhibitors of the Cip/Kip or INK family of proteins (1). In growing cells, the inactive ternary complex of cyclin D/cdk4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to effect progression through the restriction point in a pRb-dependent entry into S-phase (2). The active complex of cyclin D/cdk4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1Sphase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of its protein expression and through phosphorylation-dependent degradation (4). Although the Dtype cyclins are not fully redundant, cyclin D3, like D1, plays a prominent role in differentiation as well as a proliferative role, which correlates with higher expression levels of cyclin D3 in various cancers (5).
Catalog #C8454-20A
ApplicationsSuitable for use in Western Blotting, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern blotting 1:2000
Immunohistochemistry (Paraffin): 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3i12
HostMouse
SourceHuman
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol, less than 0.02% sodium azide.
ImmunogenRecombinant cyclin D3 of human origin and maps to residues 241-260.
SpecificityDetects endogenous levels of cyclin D3. Does not crossreact with cyclin D1 or cyclin D2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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