Anti -Cyclin D3 (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S specific cyclin E,
CCND3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
|
B IH |
|
| Activity of the cyclin-dependent kinases cdk4 and cdk6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with cdk inhibitors of the Cip/Kip or INK family of proteins (1). In growing cells, the inactive ternary complex of cyclin D/cdk4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to effect progression through the restriction point in a pRb-dependent entry into S-phase (2). The active complex of cyclin D/cdk4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1Sphase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of its protein expression and through phosphorylation-dependent degradation (4). Although the Dtype cyclins are not fully redundant, cyclin D3, like D1, plays a prominent role in differentiation as well as a proliferative role, which correlates with higher expression levels of cyclin D3 in various cancers (5). | | | Catalog # | C8454-20A | | Applications | Suitable for use in Western Blotting, Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western blotting 1:2000 | | Immunohistochemistry (Paraffin): 1:200 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 3i12 | | Host | Mouse | | Source | Human | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol, less than 0.02% sodium azide. | | Immunogen | Recombinant cyclin D3 of human origin and maps to residues 241-260. | | Specificity | Detects endogenous levels of cyclin D3. Does not crossreact with cyclin D1 or cyclin D2. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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