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Anti -Cyclin D3 (BSA & Azide Free) (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S specific cyclin E, CCND3)
Anti -Cyclin D3 (BSA & Azide Free) (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S
specific cyclin E, CCND3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH FC IF N |
|
| Applications: Flow Cytometry, Immunofluorescence, Immunoprecipitation (will not IP denatured antigen), Western Blotting, Immunohistochemistry (frozen and methanol/Carnoy’s or formalin/paraffin). Not recommended for Kinase Assay or Neutralization. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. | | | Recommended Dilution: | | | Immunoprecipitation: Use Protein G; 2ug antibody/mg protein lysate | | | Western Blotting: 1ug/ml | | | Immunohistochemistry: 2-4ug/ml | | | Optimal dilutions to be determined by the researcher. | | | Catalog # | C8454-20X | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 0.N.242 | | Host | Mouse | | Source | Human | | Concentration | 1mg/ml | | Form | Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. | | Also available with BSA and azide. See C8454-20X. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Purified human recombinant full length cyclin D3 protein. | | Specificity | Recognizes Cyclin D3 at 34kDa. Species Reactivity: Human, Monkey, Mouse and Rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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