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You are here:Home » Antibodies » Antibodies-Cyclin » Anti -Cyclin D3 (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S specific cyclin E, CCND3)

Anti -Cyclin D3 (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S specific cyclin E,
CCND3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Cyclin D3 is a G1 cyclin closely related to cyclin D1 and D2. D3 can phosphorylate pRB when associated with cdk4 and/or cdk6.
Catalog #C8454-25
ApplicationsSuitable for use in Immunoprecipitation and Western Blot Other applications not tested.
Recommended DilutionsImmunoprecipitation (Native): 2ug/mg of protein lysate. Use Protein G.
Western Blot: 1-2ug/ml for 2 hours at RT.
Optimal dilutions to be determined by the researcher.
Positive ControlsLs174T and PC12 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.243
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C8454-25X.
PurityPurified by Protein G affinity chromatography
ImmunogenPurified human recombinant full length cyclin D3 protein. Cellular Localization: Nuclear
SpecificityRecognizes human Cyclin D3 at ~34kD. Species Crossreactivity: rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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