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You are here:Home » Antibodies » Antibodies-Cyclin » Anti -Cyclin E

Anti -Cyclin E


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Clone Host Grade Applications
Monoclonal Mouse Supernatant IP IH
Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues, suggesting a post-transcriptional regulation of cyclin E. In vitro analyses indicated that these truncated variant isoforms of cyclin E are able to phosphorylate histone H1. Alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Catalog #C8455-01B
ApplicationsSuitable for use in Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (Native and Denatured): 10ul/mg protein lysate. Use Protein A.
Immunohistochemistry(Formalin/paraffin): 1:20 for 90 minutes at RT. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by researcher.
Positive ControlSKBR3 and LS174T cells, breast carcinoma or placenta.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No5G89
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 0.09% sodium azide, 40% glycerol.
ImmunogenRecombinant human cyclin E protein. Cellular Localization
SpecificityRecognizes human Cyclin E.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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