Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant IP IH

Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues, suggesting a post-transcriptional regulation of cyclin E. In vitro analyses indicated that these truncated variant isoforms of cyclin E are able to phosphorylate histone H1. Alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Catalog #	C8455-01B
Applications	Suitable for use in Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunoprecipitation (Native and Denatured): 10ul/mg protein lysate. Use Protein A.
	Immunohistochemistry(Formalin/paraffin): 1:20 for 90 minutes at RT. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
	Optimal dilutions to be determined by researcher.
Positive Control	SKBR3 and LS174T cells, breast carcinoma or placenta.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	5G89
Host	Mouse
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid in PBS, 0.09% sodium azide, 40% glycerol.
Purity	Supernatant
Immunogen	Recombinant human cyclin E protein. Cellular Localization
Specificity	Recognizes human Cyclin E.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.