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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin E (Cyclin-E, CCNE, Cyclin E1, CCNE 1, CCNE1, Cyclin Es, Cyclin Et, G1/S Specific Cyclin E)

Anti -Cyclin E (Cyclin-E, CCNE, Cyclin E1, CCNE 1, CCNE1, Cyclin Es, Cyclin Et, G1/S
Specific Cyclin E)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH IF
Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues, suggesting a post-transcriptional regulation of cyclin E. In vitro analysis indicated that these truncated variant isoforms of cyclin E are able to phosphorylate histone H1. Alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Catalog #C8455-01N
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlSKBR3, MAD109 cells, breast carcinoma, placenta
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminus of human Cyclin E.
SpecificityRecognizes human Cyclin E. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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