| Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers. |
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| Catalog # | C8455-05 |
| Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 0.5-2ug/ml detects cyclin E (~52kD) in 20ug of human A431 lysate. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C8455-05 (0.5ug/ml). Proteins were visualized by using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Immunoprecipitation: 4ug immunoprecipitated cyclin E from 500ug of human A431 RIPA cell lysate as confirmed by Western blot analysis of the immunoprecipitate using rabbit polyclonal anti-cyclin E. |
| Immunocytochemistry: 10ug/ml detected cyclin E in 50% ethanol/50% acetic acid-fixed human A431 cells. |
| Optimal dilutions to be determined by the researcher. |
| Positive Antigen Control (Included) | A0001-50 (Non-stimulated A431 cell lysate). |
| I*Recommended secondary antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| 1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo |
| I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 0.T.206 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Recombinant human Cyclin E. |
| Specificity | Recognizes human p52 cyclin E. Crossreactivity with mouse and rat was not observed. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
