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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin E, p52

Anti -Cyclin E, p52

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IC
Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Catalog #C8455-05
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detects cyclin E (~52kD) in 20ug of human A431 lysate. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C8455-05 (0.5ug/ml). Proteins were visualized by using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitated cyclin E from 500ug of human A431 RIPA cell lysate as confirmed by Western blot analysis of the immunoprecipitate using rabbit polyclonal anti-cyclin E.
Immunocytochemistry: 10ug/ml detected cyclin E in 50% ethanol/50% acetic acid-fixed human A431 cells.
Optimal dilutions to be determined by the researcher.
Positive Antigen Control (Included)A0001-50 (Non-stimulated A431 cell lysate).
I*Recommended secondary antibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo
I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No0.T.206
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human Cyclin E.
SpecificityRecognizes human p52 cyclin E. Crossreactivity with mouse and rat was not observed.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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