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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH FC IF

Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Catalog #	C8455-10
Applications	Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Other applications have not been tested.
Recommended Dilution	Western Blot: 1-2ug/ml for 2 hrs at RT.
	Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT.
	Note: Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
	Optimal dilutions to be determined by the researcher.
Positive Control	SKBR3 or LS174T cells, breast carcinomas or placenta.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.N.244
Host	Mouse
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.9% sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide. See Cat#C8455-10X.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant human cyclin E protein. Cellular Localization: Nuclear.
MW of Antigen	50kD (doublet) and 42kD (singlet)
Specificity	Recognizes human Cyclin E. Does not react with mouse or rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.