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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin E (BSA & Azide Free)

Anti -Cyclin E (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC IF
Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Catalog #C8455-10X
ApplicationsSuitable for use in Flow Cytometry, Immunofluorescence, Western Blot and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2 hours at RT. A doublet of 50kD and a single band of 42kD are observed.
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlSKBR3 or LS174T cells. Breast carcinomas or placenta.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.244
HostMouse
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See C8455-10.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human cyclin E protein. Cellular Localization: Nuclear
SpecificityRecognizes human Cyclin E. Does not react with mouse or rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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