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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP Gel

Cyclin E is a cell cycle protein, 50kD, that complexes with cdk2 and is required for DNA synthesis (1). Cyclin E/cdk2 activity peaks just before S phase. Cyclin E expression is mediated by E2F and is cleared by the ubiquitin proteolytic pathway after phosphorylation. Alterations in cyclin E expression in cancer has been correlated with increasing stage and grade of tumor. These results suggest that cyclin E may be useful as a prognostic marker for breast cancer (2,3) and gastric cancer (4), and non-small cell lung cancer (5).
Catalog #	C8455-21
Applications	Suitable for use in Western Blot, Immunoprecipitation and Gel Mobility Shift Assay. Other applications not tested.
Recommended Dilution	Western Blot: 1-2ug/ml
	Immunoprecipitation: 1ug/IP
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b,k
Clone No	3G116
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Full-length, recombinant, human cyclin E protein.
Specificity	Recognizes human cyclin E. The epitope is located within the last 80aa of the C-terminal. Detects a ~50kD band on human HeLa cell lysates by Western Blot analysis.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.