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You are here:Home » Antibodies » Antibodies-Cyclin » Anti -Cyclin E

Anti -Cyclin E


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Cyclin E is a cell cycle protein, 50kD, that complexes with cdk2 and is required for DNA synthesis (1). Cyclin E/cdk2 activity peaks just before S phase. Cyclin E expression is mediated by E2F and is cleared by the ubiquitin proteolytic pathway after phosphorylation. Alterations in cyclin E expression in cancer has been correlated with increasing stage and grade of tumor. These results suggest that cyclin E may be useful as a prognostic marker for breast cancer (2,3) and gastric cancer (4), and non-small cell lung cancer (5).
Catalog #C8455-21
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Gel Mobility Shift Assay. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Immunoprecipitation: 1ug/IP
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No3G116
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenFull-length, recombinant, human cyclin E protein.
SpecificityRecognizes human cyclin E. The epitope is located within the last 80aa of the C-terminal. Detects a ~50kD band on human HeLa cell lysates by Western Blot analysis.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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