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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin E

Anti -Cyclin E

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC
Cyclin E is a cell cycle protein that complexes with cdk2 and is required for DNA synthesis (2) . Cyclin E/cdk2 activity peaks just before S phase. Cyclin E expression is mediated by E2F and is cleared by the ubiquitin proteolytic pathway after phosphorylation. Alterations in cyclin E expression in cancer has been correlated with increasing stage and grade of tumor. These results suggest that cyclin E may be useful as a prognostic marker for breast cancer (3,4) and gastric cancer (5) , and non-small cell lung cancer (6) .
Catalog #C8455-22
ApplicationsSuitable for use in Flow Cytometry, Western Blotting, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blotting (1) : 1 2ug/ml
Immunoprecipitation (1) : 1ug/IP
Flow cytometry (8,9) :
Immunohistochemistry (10)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3G117
HostMouse
SourceHuman
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenFull-length, recombinant, human cyclin E protein.
SpecificitySpeci c for cyclin E. The epitope is located within the last 80 aa of the C-terminal (1) . HE12 antibody detects a ~50kD band on human HeLa cell lysates by Western blot analysis. Reacts with human cyclin E (HeLa cells). Reactivity with other species has not been tested. USAGE The dilutions listed below are good starting points. However, optimal dilutions should be determined by the investigator for each application. Western Blotting (1)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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