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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin E

Anti -Cyclin E

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IF
Cyclin E is a regulatory subunit of cdk-2 and is thought to be rate limiting for the G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues, suggesting a post-transcriptional regulation of cyclin E. In vitro analyses indicated that these truncated variant isoforms of cyclin E are able to phosphorylate histone H1. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers. Ab-1 is superb for biochemical applications including co-precipitation of cdk2.
Catalog #C8455-25
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Kinase Assay. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (Native and denatured): 10ug/mg protein lysate. Co-precipitates cdk2
Western Blot: 5-10ug/ml for 2hrs at RT
Optimal dilutions to be determined by the researcher.
Positive ControlMAD109 and SKBR3 cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 15mM sodium azide, 0.2% BSA. Also supplied without BSA and sodium azide. See C8455-25X.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic prptide corresponding to the C-terminal of human cyclinE. Cellular Localization
SpecificityRecognizes human Cyclin E. Species Crossreactivity: Mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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