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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E B

Cyclin E is a regulatory subunit of cdk-2 and is thought to be rate limiting for the G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues, suggesting a post-transcriptional regulation of cyclin E. In vitro analyses indicated that these truncated variant isoforms of cyclin E are able to phosphorylate histone H1. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers. Ab-1 is superb for biochemical applications including co-precipitation of cdk2.
Catalog #	C8455-25A
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:2000
	Western Blot: 1:200-1:500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid, 0.01% sodium azide.
Purity	Serum
Immunogen	Full length synthetic peptide corresponding to the human gene sequence.
Specificity	Recognizes human Cyclin E at ~45kD. Species Crossreactivity: rat, mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.