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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin E (BSA & Azide Free)

Anti -Cyclin E (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IF
Cyclin E is a regulatory subunit of cdk-2 and is thought to be rate limiting for the G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues, suggesting a post-transcriptional regulation of cyclin E. In vitro analyses indicated that these truncated variant isoforms of cyclin E are able to phosphorylate histone H1. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers. For biochemical applications including co-precipitation of cdk2.
Catalog #C8455-25X
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Kinase Assays, and Western Blot. Other applications not tested.
Recommended DilutionImmunoprecipitation: (Co-precipitates cdk2, Use Protein A; Ab at 10ug/mg protein lysate)
Western Blot: Ab at 5-10ug/ml for 2hrs at RT.
Immunohistochemistry (formalin/paraffin): 10-20ug/ml for 30 minutes at room temperature.
Note: Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Cellular LocalizationNuclear
Positive ControlMAD109 and SKBR3 cells. Breast carcinoma or placenta.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4.
Also available with BSA and azide. See C8455-25X.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH peptide conjugate corresponding to aa 381-395 (Cys-PPQSGKKQSSGPEMA) from the C-terminal of human cyclin E.
SpecificityRecognizes human Cyclin E. Species crossreactivity: Human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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