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You are here:Home » Antibodies » Antibodies-Cyclin » Anti -Cyclin E1 (CCNE, G1/S-specific Cyclin-E1)

Anti -Cyclin E1 (CCNE, G1/S-specific Cyclin-E1)


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Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IC FC
Cyclins are, as their name suggests, expressed at specific times during the cell cycle, and play a crucial role in the regulation of the cell cycle. Cyclins associate with cyclin-dependent kinases (cdks), and the association is absolutely required for cdk activity. At the conclusion of the relevant phase of the cell cycle, cyclins are rapidly ubiquitinated and degraded, thus returning cdks to their inactive state. Cyclin A associates with cdk2, and together with cyclin E is involved in the G1/S transition. Cyclin A/cdk2 remains active during S-phase. Cyclin B associates with cdk1/cdc2 to regulate the G2/M boundary. The D-cyclins bind cdk4 and cdk6, and result in Rb phosphorylation. The cyclin D1 gene was found in chronic B cell lymphocytic leukemia, and was designated Bcl-1 in a previously used nomenclature.
Catalog #C8455-26H
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:20
Western Blot: 1:1000-1:2000
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:80
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa whole cell lysate.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9H224
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
ImmunogenSynthetic peptide identical to an internal region of human Cyclin E1.
SpecificityRecognizes human Cyclin E1. Not expected to react with mouse or rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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