| Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells. |
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| Catalog # | C8850 |
| Applications | Suitable for use in Western Blot. Other applications have not been tested. |
| Recommended Dilution | Western Blot: 1:1000-1:10,000 detects Cyclophilin A in RIPA lysates from nonstimulated A431 cells. A previous lot detected Cyclophilin A in RIPA lysates from rat lung cells. 20ug of A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C8850 (1:10,000). Proteins were visualized using a Gt xRb IgG (HRP)* and a chemiluminescence detection system. |
| Optimal dilutions to be determined by the researcher. |
| *Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Included Positive Antigen Control | A0001-50: Non stimulated A431 cell lysate |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol. |
| Purity | Serum |
| Immunogen | Recombinant full length protein corresponding to human cyclophilin A. |
| Specificity | Recognizes human cyclophilin A (18kD). Additional bands may be detected at higher concentrations of the antibody. Species Crossreactivity: rat, mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
