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You are here:Home » Antibodies » Abs to Cyclophilin » Anti -Cyclophilin B (PPIB, Peptidylpropyl Isomerase B)

Anti -Cyclophilin B (PPIB, Peptidylpropyl Isomerase B)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IC
Cyclophilin B is a 24kD glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, Cyclophilin B mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. The human Cyclophilin B precursor is 216 amino acids (aa) in length. It contains a 25aa signal sequence plus a 191aa mature region. There is a partial heparin-binding sequence (aa27-34), a PPIase domain (aa47-204) and a C-terminal ER retention motif (aa213-216).
Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Catalog #C8855-01B
Recommended DilutionWestern Blot: 1ug/ml detects Cyclophilin B in HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line and NRK rat normal kidney cells.
Immunocytochemistry: 8-25ug/ml. Immersion fixed HeLa human cervical epithelial carcinoma cell line
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9H108
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to Asp34-Glu216 from human Cyclophilin B expressed in E. coli (Accession #P23284).
Species Sequence HomologyMouse (95%).
SpecificityRecognizes human Cyclophilin B. Does not crossreact with recombinant human Cyclophilin A. Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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