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You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -Cystatin A (Stefin A, Cystatin-A, Cystatin-AS, Stefin-A, CSTA, STF1, STFA)

Anti -Cystatin A (Stefin A, Cystatin-A, Cystatin-AS, Stefin-A, CSTA, STF1, STFA)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH
Stefin A is the intracellular inhibitor of cysteine proteinase cathepsin B (CatB) and belongs to family 1 of the cystatin superfamily of inhibitor proteins. It has been proposed that extracellular CatB together with other classes of proteinases are all subject to a cascade-like manner of activation. CatB and its inhibitor Stefin A are involved in degradation of extracellular matrix proteins during tissue remodeling. Stefin A had been reported to be in high concentrations in epithelial cells, polymorphonuclear leukocytes and lymphoid tissue.
Catalog #C8949-18E
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 1:200 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min.
Optimal dilutions to be determined by the researcher.
Positive ControlAppendix
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to full length human Stefin A.
SpecificityRecognizes human Stefin A.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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