Cystatin F, Recombinant, Mouse (CMAP, CST7, Cystatin 7, Leukocystatin, Cystatin-like
Metastasis-associated Protein)
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| Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a new member of the Cystatin superfamily (1-3). Cystatin F is selectively expressed by hematopoietic cells and may be a biomarker for both liver metastasis and inflammatory lung disorders (3, 4). As a cysteine protease inhibitor, it shows selectivity towards Cathepsin L and legumain (1, 2, 5). Compared to other secreted Cystatins including C, D, E/M, S, SA and SN, which contain two intrachain disulfide bonds, Cystatin F has two extra Cys residues that may be involved in interchain disulfide bonds. rmCystatin F and rhCystatin F exhibit disulfide bond-linked dimer formation, which was also the case for an E. coli expressed fusion protein containing mature human Cystatin F and glutathione S-transferase (2). | | | Catalog # | C8950-22D | | Recombinant mouse Cystatin F encoded by the Cst7 gene (rmCystatin F; amino acid residues 19 to 144; Accession # O89098) was expressed with an N-terminal signal sequence and a C-terminal 6X His tag in a murine myeloma cell line, NS0. | | Calculated molecular mass of 15kD and migrates as a 20kD band in SDS-PAGE under reducing conditions. | | Activity | Measured by its ability to inhibit human cathepsin L. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Source | Recombinant Mouse | | Purity | 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. | | Endotoxin | 1EU/1ug. | | Concentration | As reported | | Form | Supplied as a liquid in 25mM Tris, 0.15M NaCl, pH 7.5. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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