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Anti -Cysteine, S-Nitrosylated (SNO-Cys)
Anti -Cysteine, S-Nitrosylated (SNO-Cys)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH |
|
| Nitric oxide (NO), generated by cell type-specific NO-synthase (NOS) isoforms, is a freely diffusible intercellular messenger that functions in target cells in NOS-dependent signaling. S-nitrosylation of cysteine thiols in proteins by the highly labile NO radical has been identified as an important effector of NO-related bioactivity both in NOS-containing cells and intercellular signaling. Most cells contain low levels of nitrosylated proteins that are thought to be regulated by S-nitrosylation and denitrosylation. S-nitrosylation of proteins serves as a ubiquitous post-translational modification that dynamically regulates a broad functional spectrum of proteins. The majority of these proteins are regulated by S-nitrosylation on a single critical cysteine residue within an acidic/basic or hydrophobic structural motif that may also be subject to oxygen- or glutathione-dependent modification. NO-sensitive ion channels including the cardiac and skeletal muscle ryanodine receptor (RyR1), N-methyl-D-aspartate receptor (NMDAR) complex, cyclic-nucleotide gated ion channel, are modulated by S-nitrosylation. S-nitrosylation of capsase-3 inhibits apoptosis signaling. S-nitrosylation activates matrix metalloproteinase-9 (MMP-9) and induces neuronal apoptosis. The small G-protein p21Ras and Jun kinase are regulated by S-nitrosylation. The activity of transcription factors such as NFkB, c-jun, and c-fos is modulated by S-nitrosylation. In addition, the formation of S-nitrosylated glutathione (GSNO) has been proposed to be one of the major storage forms of NO in vivo. | | | Catalog # | C9002-75 | | Applications | Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications not tested. | | Recommended Dilutions | Immunohistochemistry: 2-10ug/ml. Use bovine endothethial cells treated with Ca+2 ionophore A23187 as a control. | | Western Blot: 1:500-1:2000 using nitrosylated-Cysteine–BSA (C9002-75A) as a control. | | ELISA: 1:1000-1:5000; using 50-100ng control proteins/well. | | Optimal dilution to be determined by researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Concentration | ~1mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Nitrosylated-Cystein, conjugated to KLH (hemocyanin). | | Specificity | Recognizes nitrosylated-cysteine modified proteins from all species. No significant reactivity is observed with unmodified BSA or Cysteine-BSA (not nitrosylated). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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