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You are here:Home » Biochemicals » Biochem-Amino Acids, Derivatives » Cysteine, S-Nitrosylated (SNO-Cys) (BSA)

Cysteine, S-Nitrosylated (SNO-Cys) (BSA)


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Description: Nitric oxide (NO), generated by cell type-specific NO- synthase (NOS) isoforms, is a freely diffusible intercellular messenger that functions in target cells in NOS-dependent signaling. S-nitrosylation of cysteine thiols in proteins by the highly labile NO radical has been identified as an important effector of NO-related bioactivity both in NOS- containing cells and intercellular signaling. Most cells contain low levels of nitrosylated proteins that are thought to be regulated by S-nitrosylation and denitrosylation. S-nitrosylation of proteins serves as a ubiquitous post- translational modification that dynamically regulates a broad functional spectrum of proteins. The majority of these proteins are regulated by S-nitrosylation on a single critical cysteine residue within an acidic/basic or hydrophobic structural motif that may also be subject to oxygen- or glutathione-dependent modification. NO- sensitive ion channels including the cardiac and skeletal muscle ryanodine receptor (RyR1), N-methyl-D-aspartate receptor (NMDAR) complex, cyclic-nucleotide gated ion channel, are modulated by S-nitrosylation. S-nitrosylation of capsase-3 inhibits apoptosis signaling. S-nitrosylation activates matrix metalloproteinase-9 (MMP-9) and induces neuronal apoptosis. The small G-protein p21Ras and Jun kinase are regulated by S-nitrosylation. The activity of transcription factors such as NFB, c-jun, and c-fos is modulated by S-nitrosylation. In addition, the formation of S-nitrosylated glutathione (GSNO) has been proposed to be one of the major storage forms of NO in vivo.
SNO-Cys-BSA protein conjugate was prepared by coupling Cysteine to BSA followed by nitrosylation of cysteines.  
Catalog #C9002-75A
ApplicationsSuitable for use in ELISA and Antibody Blocking. Other applications not tested.
Recommended DilutionAntibody Blocking: 10ug/1ug C9002-75.
ELISA: 1:1000-1:5000, 50-100ng control protein/welll.
Optimal dilutions to be determined by the researcher.
Storage and StabilityAliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
FormSupplied as a liquid in PBS, pH 7.2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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