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Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)
Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IP IC |
|
| Catalog # | C9040-01 | | Applications | Suitable for use in Immunofluorescence, ELISA, Western Blotting, Immunoprecipitation, Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Immunoblot Analysis: 0.1–0.5ug/ml detects CFTR in RIPA lysates from CFTR-transfected BHK cells. Reported to detect CFTR from a human T84 colon carcinoma epithelial cell lysate. CFTR-transfected BHK cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti- CFTR (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Immunoprecipitation | Immunoprecipitates CFTR from a T84 cell lysate. | | Immunocytochemistry | Reported to immunostain CFTR in T84 cells. | | Note: CFTR runs as a diffuse protein on SDS-PAGE. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 2Q999 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Synthetic peptide (RKGYRQRLELSD) corresponding to residues 25–36 of human cystic fibrosis transmembrane conductance regulator (CFTR). The immunizing sequence is identical in monkey and rabbit, has 11/12 identical amino acids in sheep, cow and frog, 10/12 identity in mouse and 9/12 identity in rat. | | Specificity | Recognizes human CFTR, Mr 170kD. Two additional proteins were detected, Mr 52 and 38kD. | | | Important Note | This product is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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