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You are here:Home » Antibodies » Antibodies-Disease Markers » Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)

Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IC
Catalog #C9040-01
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.52ug/ml detects CFTR in RIPA lysates from CFTR-transfected BHK cells. Reported to detect CFTR from a human T84 colon carcinoma epithelial cell lysate. CFTR-transfected BHK cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C9040-01 (0.5ug/ml). Proteins were visualized using IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system.
Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes.
Immunoprecipitation: Immunoprecipitates CFTR from a T84 cell lysate.
Immunocytochemistry: Reported to immunostain CFTR in T84 cells.
Note: CFTR runs as a diffuse protein on SDS-PAGE.
Optimal dilutions to be determined by the researcher.
Recommended secondary antibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No2Q999
ConcentrationAs reported
FormSupplied as a liquid in 0.02M PBS, pH 7.6, 0.25M sodium chloride, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide () corresponding to residues 25-36 RKGYRQRLELSD of human cystic fibrosis transmembrane conductance regulator (CFTR). The immunizing sequence is identical in monkey and rabbit, has 11/12 identical amino acids in sheep, bovine and frog, 10/12 identity in mouse and 9/12 identity in rat.
SpecificityRecognizes human CFTR, Mr 170kD. Two additional proteins were detected, Mr 52 and 38kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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