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You are here:Home » Antibodies » Abs to Disease Markers » Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)

Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
Catalog #C9040-02
Applications Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry and Western Blot . Other applications not tested.
Recommended DilutionImmunoprecipitation: Reported to immunoprecipitate CFTR.1
Immunohistochemistry: Reported to immunostain CFTR in human pancreatic tissue sections.1
Immunoblot Analysis: 0.52ug/ml detects CFTR from 2050ug of human T84 colon carcinoma epithelial RIPA cell lysates. 0.52ug/ml of a previous lot detected CFTR from CFTR-transfected BHK2. Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE. T84 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CFTR (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No4i35
FormSupplied as a liquid in ~530ul of 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenFusion Protein corresponding to residues 11971480 of human cystic brosis transmembrane conductance regulator (CFTR). The epitope maps within amino acids 13651395.
SpecificityRecognizes CFTR, Mr 170kDa. Species Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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