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Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)
Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IP IH |
|
| Catalog # | C9040-02 | | Applications | Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry and Western Blot . Other applications not tested. | | Recommended Dilution | Immunoprecipitation: Reported to immunoprecipitate CFTR.1 | | Immunohistochemistry: Reported to immunostain CFTR in human pancreatic tissue sections.1 | | Immunoblot Analysis: 0.5–2ug/ml detects CFTR from 20–50ug of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5–2ug/ml of a previous lot detected CFTR from CFTR-transfected BHK2. Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE. T84 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CFTR (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 4i35 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in ~530ul of 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Fusion Protein corresponding to residues 1197–1480 of human cystic brosis transmembrane conductance regulator (CFTR). The epitope maps within amino acids 1365–1395. | | Specificity | Recognizes CFTR, Mr 170kDa. Species Crossreactivity: Human. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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