Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR, CF, CFTR/MRP, ABC35,
ATP-binding Cassette Sub-family C Member 7, ATP Binding Cassette Superfamily C Member 7,
ABCC7, cAMP-dependent Chloride Channel, Channel Conductance-controlling ATPase, dJ760C
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
B IP IH |
|
| CFTR (cystic fibrosis transmembrane conductance regulator) is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation. | | | Catalog # | C9040-05D | | Applications | Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. | | Recommended Dilutions | Immunohistochemistry (Frozen): The epitope is sensitive to formaldehyde fixation and tissue processing. Acetone fixation is recommended for frozen sections. Not recommeneded for use in paraffin sections. | | Western Blot: 10ug/ml detects a band at 170kD under native conditions. Do not boil the lysate. Instead incubate at 37°C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 10B2212 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, 0.1% sodium azide. | | Purity | Purified | | Immunogen | Fusion protein containing residues 1197-1480 of human CFTR | | Specificity | Recognizes human CFTR. Recognizes an epitope in the ABC transporter 2 domain, specifically to a region between aa1370-1380. Does not react with mouse | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | ATP Binding Cassette Transporter Sub-family C Member 7, Cystic Fibrosis Transmembrane Regulator |
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