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You are here:Home » Antibodies » Abs to Disease Markers » Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR, ABCC7)

Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR, ABCC7)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IP IH FC IF
Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake.
Catalog #C9040-10
ApplicationsSuitable for use in Immunofluorescence, Flow cytometry, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:500; Immunofluorescence staining of CFTR in mouse epithelial cells results in cell surface staining, consistent with localization at the plasma membrane.
Western Blot: 1:500; Detects a single ~170kD protein representing CFTR in T84 whole cell extract.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgM
Clone No4i327
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, 0.05% sodium azide.
PurityAscites
ImmunogenSynthetic peptide corresponding to aa103-117, RIIASYDPDNKEER in the first extracellular loop of human and rabbit CFTR. Cellular Localization: Membrane; multi-pass membrane protein.
SpecificityRecognizes human CTFR. Also detects one or more immunologically related proteins in murine cell line Heb7a that does not contain CFTR mRNA. Can also be used to inhibit the epithelial uptake of S. typhi in some mouse cell lines. The peptide sequence is highly conserved in mouse, sheep, bovine and Xenopus laevis. Species Crossreactivity: mouse, rat, chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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