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Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR, ABCC7)
Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR, ABCC7)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
B IP IC |
|
| Catalog # | C9040-10A | | Applications | Suitable for use in Immunocytochemistry, Immunoprecipitation, and Western Blot. Also suitable for functional studies (inhibits epithelial uptake of S. typhi in some mouse cell lines). Other applications not tested. | | Recommended Dilution | Immunocytochemistry: 1:500. Staining of CFTR in mouse epithelial cells results in cell surface staining consistent with localization at the plasma membrane. | | Western Blot: 1:500 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgM | | Clone No | 6D334 | | Host | Mouse | | Source | Human | | Form | Liquid. Diluted ascites containing 0.05% sodium azide. | | Purity | Ascites | | Immunogen | Synthetic peptide corresponding to aa 103-117(G103RIIASYDPDNKEER117) in the first extracellular loop of human and mouse CFTR (cystic fibrosis transmembrane conductance regulator). This sequence is highly conserved in sheep, bovine and Xenopus laevis CFTR. | | Specificity | Recognizes human and mouse CFTR. Cross-reacts with immunologically related proteins in mouse cell line Heb7a that doesn not contain CFTR mRNA. | | | Detects a band of ~170kDa by Western blot. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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