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You are here:Home » Antibodies » Abs to Enzymes, Cytochrome » Anti -CYBB (Cytochrome b-245 Heavy Chain, CGD91-phox, Cytochrome b(558) Subunit beta, Cytochrome b558 Subunit beta, Heme-binding Membrane Glycoprotein gp91phox, NADPH Oxidase 2, Neutrophil Cytochrome b 91kD Polypeptide, Superoxide-generating NADPH Oxidase Heavy

Anti -CYBB (Cytochrome b-245 Heavy Chain, CGD91-phox, Cytochrome b(558) Subunit beta,
Cytochrome b558 Subunit beta, Heme-binding Membrane Glycoprotein gp91phox, NADPH Oxidase
2, Neutrophil Cytochrome b 91kD Polypeptide, Superoxide-generating NADPH Oxidase Heavy

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
The heavy chain of cytochrome B245, also known as gp91phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections. The heavy chain (gp91phox), along with the light chain (p22phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons.
Catalog #C9094-34
pplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.03-0.1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Peripheral Blood Mononucleocytes lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-SYLNFARKRIKNP, from the internal region of the protein sequence according to NP_000388.2.
SpecificityRecognizes human CYBB. Species sequence homology: canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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