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You are here:Home » Antibodies » Abs to Enzymes, Cytochrome » Anti -CYBB (Cytochrome b-245 Heavy Chain, CGD91-phox, Cytochrome b(558) Subunit beta, Cytochrome b558 Subunit beta, Heme-binding Membrane Glycoprotein gp91phox, NADPH Oxidase 2, Neutrophil Cytochrome b 91kD Polypeptide, Superoxide-generating NADPH Oxidase Heavy

Anti -CYBB (Cytochrome b-245 Heavy Chain, CGD91-phox, Cytochrome b(558) Subunit beta,
Cytochrome b558 Subunit beta, Heme-binding Membrane Glycoprotein gp91phox, NADPH Oxidase
2, Neutrophil Cytochrome b 91kD Polypeptide, Superoxide-generating NADPH Oxidase Heavy

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
The integral membrane protein flavocytochrome b (Cyt b), a heterodimer formed by a 91kD glycoprotein (heavy chain) and a 22kD polypeptide (light chain), is the catalytic core of the phagocyte NADPH oxidase and generates superoxide which plays a critical role in host defense. NADPH oxidase is a central well characterized component of the innate immune response against fungal and bacterial infections. Recent research also offers new insight into a novel role for this oxidase in the coordination of the adaptive immune response. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
Catalog #C9094-34B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:2000
Western Blot: 0.03-0.1ug/ml, observed in human peripheral blood mononucleocytes lysates on ~85kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-SYLNFARKRIKNP, from human CYBB, at the internal region of the protein (NP_000388.2).
SpecificityRecognizes human CYBB. Species sequence homology: Canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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