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You are here:Home » Antibodies » Abs to Enzymes, Cytochrome » Anti -Cytochrome B245 Heavy Chain (Cytochrome b-245 Heavy Chain, CGD, CGD91-phox, Cytochrome b(558) Subunit beta, Cytochrome b558 Subunit beta, CYBB, gp91-1, GP91PHOX, gp91-phox, Heme-binding Membrane Glycoprotein gp91phox, NADPH Oxidase 2, NOX2, Neutrophil Cyt

Anti -Cytochrome B245 Heavy Chain (Cytochrome b-245 Heavy Chain, CGD, CGD91-phox,
Cytochrome b(558) Subunit beta, Cytochrome b558 Subunit beta, CYBB, gp91-1, GP91PHOX,
gp91-phox, Heme-binding Membrane Glycoprotein gp91phox, NADPH Oxidase 2, NOX2, Neutrophil
Cyt

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
The heavy chain of cytochrome B245, also known as gp91phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections. The heavy chain (gp91phox), along with the light chain (p22phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons.
Catalog #C9094-61
ApplicationsSuitable for use in Flow cytometry. Other applications have not been tested.
Recommended Dilutions
Flow cytometry: Neat-1:10. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Membrane permeabilisation is required for this application.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1
Clone NoNL7
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide, 1% BSA. Labeled with FITC.
PurityPurified by Protein G affinity chromatography.
ImmunogenCytochrome B245 solubilised in octyl-beta-glucopyranoside.
SpecificityRecognizes the native as well as the denatured form of the heavy chain of human Cytochrome B245. Epitope: aa498-506 (EKDVITGLK). Has an inhibitory effect on NADPH oxidase activation. Research indicates that this inhibitory effect is exerted during the initial stages of oxidase activation. Does not interfere with the translocation of the cytosolic subunits of NADPH oxidase to the cell membrane.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCytochrome b-245, beta Polypeptide, EC=1.-.-.-


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