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Specifications

Catalog #	C9094-90
Principle of the Assay	The Cytochrome C ELISA kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA). A monoclonal antibody specific for Cytochrome c has been coated onto the wells of the microtiter strips provided. Samples, including a standard containing Cytochrome c, control specimens and unknowns, are pipetted into these wells. During the first incubation, the Cytochrome c antigen binds to the immobilized (capture) antibody. After washing, a biotinylated secondary antibody specific for Cytochrome c is added to the wells. During the second incubation, this antibody serves as a detection antibody by binding to the immobilized Cytochrome c protein captured during the first incubation. After removal of excess detection antibody, a Streptavidin-Peroxidase (enzyme) is added. This binds to the biotinylated detection antibody to complete the four-member sandwich. After a third incubation and washing to remove all the excess Streptavidin-Peroxidase, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Cytochrome c present in the original specimen.
Kit Components
C9094-90A	Microwell Plate, 1x96 wells Supplied as removable strips coated with mouse monoclonal antibody to Cytochrome c.
C9094-90B*	Standard, 2 x1vial Supplied as 10ng of lyophilized powder.
C9094-90C	Standard Diluent Buffer, 1x25ml Supplied as a liquid, 0.1% sodum azide, red dye**.
C9094-90D	Pab (Biotin), 1x11ml Biotin-labeled anti-Cytochrome c antibody. Supplied as a liquid, 0.1% sodium azide, blue dye**.
C9094-90E	Streptavidin (HRP) 100X, 1x125ul Supplied as a liquid, 3.3mM thymol, 50% glycerol.
C9094-90F	HRP Diluent, 1x25ml Supplied as a liquid, 3.3mM thymol, yellow dye**.
C9094-90G	Wash Buffer 25X, 1x100ml
C9094-90H	TMB Substrate, 1x25ml
C9094-90J	Stop Solution, 1x25ml
Storage and Stability	*Store reconstituted C9094-90B at -70ºC. Store all other components at 4ºC. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Analytical Sensitivity	<0.156ng/ml of Cytochrome c
Specificity	Detects human, rat and mouse forms of the Cytochrome c protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.