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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites B IH

Cytochrome C/Apaf-2 (apoptosis protease activating factor-2) is well known for its role in electron transport. During apoptosis (programmed cell death), Cytochrome C is released from the mitochondria into the cytosol where it binds to Apaf-1. Apaf-1 then activates caspase 9. Caspase 9 activation triggers other downstream caspase events in the proteolytic cascade. The mechanism by which Cytochrome C is released from the mitochondria and its function in Caspase 9 activation is still unclear.
Catalog #	C9095
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Western Blot Analysis: 1:500-1:2000 detects Cytochrome C in RIPA lysates from A431 cells. EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Cytochrome c (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
	Immunohistochemistry: Reported to stain rat spine cord sections.
	Optimal dilutions to be determined by the researcher.
Positive Antigen Control	EGF-stimulated A431 lysate. Use 20ug per lane for minigels.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Clone No	0.T.26
Host	Mouse
Source	Equine
Concentration	Not determined
Form	Supplied as mouse ascites containing 0.05% sodium azide
Purity	Ascites
Immunogen	Horse Cytochrome C (KLH coupled)
Specificity	Recognizes equine Cytochrome C at 14kD. Species Crossreactivity: human
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.