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You are here:Home » Antibodies » Abs to Enzymes, Cytochrome » Anti -Cytochrome C (CYC, Cytochrome C Somatic, CYCS, HCS, THC4)

Anti -Cytochrome C (CYC, Cytochrome C Somatic, CYCS, HCS, THC4)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Cytochrome c is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome c has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome c is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome c functions in the activation of caspase-3, and ICE family molecule that is a key effector of apoptosis.
Catalog #C9095-02
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Not suitable for Immunoprecipitation. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution determined by the researcher.
Recommended Positive ControlHeLa cells. Cardiac muscle.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No7H8.2C12
HostMouse
SourcePigeon
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide. See C9095-02X.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptides corresponding to aa1-80, 81-104 and 66-104 of pigeon cytochrome c. Cellular Localization: Cytoplasmic.
MW15kD
SpecificityRecognizes pigeon Cytochrome C. Species Crossreactivity: human, mouse, rat, equine, canine, frog, and Drosophila.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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