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You are here:Home » Antibodies » Abs to Enzymes, Cytochrome » Anti -Cytochrome C (CYC, Cytochrome C Somatic, CYCS, HCS, THC4)

Anti -Cytochrome C (CYC, Cytochrome C Somatic, CYCS, HCS, THC4)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
In addition to its well-known role in electron transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death (1). Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade by activating caspase-3 (2). Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3 (3).
Catalog #C9095-02A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:250-1:500
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H8.2C12
HostMouse
SourcePigeon
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptides corresponding to aa1-80, 81-104 and 66-104 of pigeon CYT. Epitope: Within aa93-104 of pigeon CYT.
SpecificityRecognizes CYT (~15kD). Species Crossreactivity: Human, mouse, rat, pigeon, hamster, equine and Drosophila.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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