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You are here:Home » Antibodies » Abs to Enzymes, Cytochrome » Anti -Cytochrome C (CYC, HCS, THC4)

Anti -Cytochrome C (CYC, HCS, THC4)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Cytochrome C is a 15kD protein in mitochondria which functions as an electron carrier in the cell respiratory chain by interacting with redox partners of complex III and complex IV. Cytochrome C is highly conserved during evolution. Recent evidence shows that Cytochrome C translocates from mitochondria to the cytosol in the cells undergoing apoptosis and participates in the central control or executioner phase of cell death cascade. Caspase-9 (Apaf-3) and Apaf-1 bind to each other in the presence of Cytochrome C and dATP, an event that leads to Caspase-9 activation. This activation, along with Cytochrome C, plays a major role in the activation of DEVD-specific caspases such as Caspase-3 and DNA fragmentation. Apoptosis inhibitors, Bcl-2 or Bcl-xL, prevent the efflux of Cytochrome C from the mitochondria and Bax, a pro-apoptotic member of the Bcl-2 protein family, can induce Cytochrome C release.
Catalog #C9095-02J
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
Immunohistochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa, COS, and L929 cell lysates
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9H225
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues near the N-terminus of human Cytochrome C. Cellular Localization
SpecificityRecognizes human Cytochrome C.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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