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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP IH IC

Cytochrome C is a 15kD protein in mitochondria which functions as an electron carrier in the cell respiratory chain by interacting with redox partners of complex III and complex IV. Cytochrome C is highly conserved during evolution. Recent evidence shows that Cytochrome C translocates from mitochondria to the cytosol in the cells undergoing apoptosis and participates in the central control or executioner phase of cell death cascade. Caspase-9 (Apaf-3) and Apaf-1 bind to each other in the presence of Cytochrome C and dATP, an event that leads to Caspase-9 activation. This activation, along with Cytochrome C, plays a major role in the activation of DEVD-specific caspases such as Caspase-3 and DNA fragmentation. Apoptosis inhibitors, Bcl-2 or Bcl-xL, prevent the efflux of Cytochrome C from the mitochondria and Bax, a pro-apoptotic member of the Bcl-2 protein family, can induce Cytochrome C release.
Catalog #	C9095-02J
Applications	Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:500
	Immunohistochemistry: 1:100-1:250
	Optimal dilutions to be determined by the researcher.
Positive Control	HeLa, COS, and L929 cell lysates
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9H225
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Purity	Supernatant
Immunogen	Synthetic peptide corresponding to residues near the N-terminus of human Cytochrome C. Cellular Localization
Specificity	Recognizes human Cytochrome C.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.