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You are here:Home » Antibodies » Abs to Enzymes, Cytochrome » Anti -Cytochrome C (CYCS, CYC)

Anti -Cytochrome C (CYCS, CYC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IC FC IF
Cytochrome c is a 15kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins.
Catalog #C9095-02K
ApplicationsSuitable for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence and Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
HostMouse
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRat cyt c-OVA
SpecificityRecognizes rat Cytochrome c. Species Crossreactivity: mouse and human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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