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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IP IC FC IF

Cytochrome c is a 15kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins.
Catalog #	C9095-02K
Applications	Suitable for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Flow Cytometry: 0.5ug/10e6 cells in 100ul volume
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG1,k
Host	Mouse
Source	Rat
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Rat cyt c-OVA
Specificity	Recognizes rat Cytochrome c. Species Crossreactivity: mouse and human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.