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C9095-02K Mouse Anti-Cytochrome C (CYCS, CYC)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Rat
Swiss Prot
P62898
Isotype
IgG1,k
Clone Number
6H2.B4
Grade
Affinity Purified
Applications
FC IC IF IP
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cytochrome c is a 15kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins.

Applications
Suitable for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Flow Cytometry: ≤0.5ug/10e6 cells in 100ul volume Western Blot: 1:1000 Immunoprecipitation: 2-4ug/1x10e7 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Rat cyt c-OVA
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes rat Cytochrome c. Species Crossreactivity: mouse and human.
References
1. Liu X, et al. 1996. Cell. 86:147. 2. Li P, et al. 1997. Cell. 91:479. 3. Zhang Z, et al. 2003. Gene 312:61. 4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.
USBio References
No references available
Conjugates
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