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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B

Cytochrome c is a 15kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins.
Catalog #	C9095-02L
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 2ul/ml dilution buffer for each mini-gel
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Host	Mouse
Concentration	As reported
Form	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Equine cyt c-OVA
Specificity	Recognizes Cytochrome c. Species Crossreactivity: broad
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.