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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IP FC

Cytochrome c is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome c has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome c is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome c functions in the activation of caspase-3, and ICE family molecule that is a key effector of apoptosis.
Catalog #	C9095-03X
Applications	Suitable for use in ELISA, Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions	ELISA: For coating
Immunoprecipitation (Use Protein A)	2ug/mg protein lysate.
	Optimal dilution determined by the researcher.
Positive Control	HeLa cells.
Cellular Localization	Cytoplasmic
MW of Antigen	15kD
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	3F144
Host	Mouse
Source	Equine
Concentration	~1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See C9095-03.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Equine cytochrome c.
Specificity	Recognizes Cytochrome c
Species Crossreactivity	Human, bovine, equine and canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.