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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IP IF

Cytochrome c is a requisite component of the mitochondrial respiratory chain and participates in the electron transfer between complex III and complex IV of the respiratory chain. Cytochrome c is a soluble protein that is localized in the mitochondrial intermembrane space and is loosely attached to the surface of the inner membrane. The protein is synthesized on cytoplasmic ribosomes as apocytochrome c and then translocated into the mitochondria via a unique pathway independent of the general protein translocation machinery, protease-sensitive components of the outer membrane, or a membrane potential across the inner membrane. In response to a variety of apoptotic stimuli, Cytochrome c is released from mitochondria into the cytosol. This release can be blocked by Bcl-2 and suggests that Bcl-2 may function by regulating Cytochrome c release. Cytosolic Cytochrome c is an essential component of the vertebrate apoptosome which is composed of Cytochrome c, Apaf-1, and procaspase-9 (Apaf-3). Interaction of these proteins leads to the activation of caspase-9, which in turn activates other caspases (such as caspase-3) ultimately resulting in cell death. The current working hypothesis is that release of Cytochrome c from mitochondria commits a cell to die either by a “rapid” apoptotic mechanism involving activation of the Apaf-1/caspase-9 cascade, or by a “slower” necrotic process involving the collapse of the electron transport chain.
Catalog #	C9095-04A
Applications	Suitable for use in Immunofluorescence, ELISA and Immunoprecipitation. Other applications not tested.
Recommended Dilution	ELISA (Native): 0.1-1ug/ml
	Immuno uorescence: 20ug/ml
	Immunoprecipitation (Native): 15ug (for extract from ~10e6 cells)
	Optimal dilutions to be determined by the researcher.
	Positive Control:
	HeLa, Rat6
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1b,k
Clone No	3G119
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Rat cytochrome C.
Specificity	Recognizes native rat Cytochrome C, but does not recognize denatured Cytochrome C. Recognizes a region in the vicinity of aa62 of rat Cytochrome C. Species Crossreactivity: Human, mouse, rabbit and Drosophila. Not tested for Immunofluorescence in rabbit and Drosophila.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.